Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

authored by

Mireia Pesarrodona, Toni Jauset, Zamira V. Díaz-Riascos, Alejandro Sánchez-Chardi, Marie Eve Beaulieu, Joaquin Seras-Franzoso, Laura Sánchez-García, Ricardo Baltà-Foix, Sandra Mancilla, Yolanda Fernández, Ursula Rinas, Simó Schwartz, Laura Soucek, Antonio Villaverde, Ibane Abasolo, Esther Vázquez

Abstract

Two structurally and functionally unrelated proteins, namely Omomyc and p31, are engineered as CD44-targeted inclusion bodies produced in recombinant bacteria. In this unusual particulate form, both types of protein materials selectively penetrate and kill CD44⁺ tumor cells in culture, and upon local administration, promote destruction of tumoral tissue in orthotropic mouse models of human breast cancer. These findings support the concept of bacterial inclusion bodies as versatile protein materials suitable for application in chronic diseases that, like cancer, can benefit from a local slow release of therapeutic proteins.

Organisation(s)

Institute of Technical Chemistry

External Organisation(s)

IRB Barcelona - Institute for Research in Biomedicine
Vall d'Hebron Barcelona Campus Hospital
Universitat de Barcelona
Helmholtz Centre for Infection Research (HZI)
Vall d’Hebron Institute of Oncology (VHIO)
Autonomous University of Barcelona (UAB)
Centros de Investigacion Biomedica en Red - CIBER

Type

Article

Journal

Advanced Science

Volume

6

ISSN

2198-3844

Publication date

18.09.2019

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Medicine (miscellaneous), General Chemical Engineering, Biochemistry, Genetics and Molecular Biology (miscellaneous), General Materials Science, General Engineering, General Physics and Astronomy

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1002/advs.201900849 (Access: Open)
https://doi.org/10.15488/5232 (Access: Open)