Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering

authored by: M. Granados, L. Morticelli, S. Andriopoulou, P. Kalozoumis, M. Pflaum, P. Iablonskii, Birgit Glasmacher, M. Harder, Jan Hegermann, C. Wrede, I. Tudorache, S. Cebotari, A. Hilfiker, A. Haverich, Sotirios Korossis
Abstract: Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
Organisation(s): Institute of Multiphase Processes
External Organisation(s): Hannover Medical School (MHH)
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO)
Corlife OHG
Type: Article
Journal: Journal of Cardiovascular Translational Research
Volume: 10
Pages: 374-390
No. of pages: 17
ISSN: 1937-5387
Publication date: 08.2017
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Molecular Medicine, Genetics, Pharmaceutical Science, Cardiology and Cardiovascular Medicine, Genetics(clinical)
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Electronic version(s): https://doi.org/10.1007/s12265-017-9747-z (Access: Closed)