Guidelines for small-scale production and purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris
- authored by
- Maria Zahid, Ursula Rinas
- Abstract
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
- Organisation(s)
-
Institute of Technical Chemistry
- External Organisation(s)
-
Lahore University of Management Sciences (LUMS)
Helmholtz Centre for Infection Research (HZI)
- Type
- Contribution to book/anthology
- Pages
- 309-322
- No. of pages
- 14
- Publication date
- 09.02.2019
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Molecular Biology, Genetics
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Electronic version(s)
-
https://repository.helmholtz-hzi.de/handle/10033/621706 (Access:
Open)
https://doi.org/10.1007/978-1-4939-9024-5_14 (Access: Closed)
