Possibilities and efficiency of MSC co-transfection for gene therapy
- authored by
- Sina Christoffers, Lisa Seiler, Elena Wiebe, Cornelia Blume
- Abstract
Mesenchymal stem/stromal cells (MSCs) are not only capable of self-renewal, trans-differentiation, homing to damaged tissue sites and immunomodulation by secretion of trophic factors but are also easy to isolate and expand. Because of these characteristics, they are used in numerous clinical trials for cell therapy including immune and neurological disorders, diabetes, bone and cartilage diseases and myocardial infarction. However, not all trials have successful outcomes, due to unfavourable microenvironmental factors and the heterogenous nature of MSCs. Therefore, genetic manipulation of MSCs can increase their prospect. Currently, most studies focus on single transfection with one gene. Even though the introduction of more than one gene increases the complexity, it also increases the effectivity as different mechanism are triggered, leading to a synergistic effect. In this review we focus on the methodology and efficiency of co-transfection, as well as the opportunities and pitfalls of these genetically engineered cells for therapy. Graphical abstract: (Figure presented.)
- Organisation(s)
-
Institute of Technical Chemistry
- External Organisation(s)
-
Cluster of Excellence Hearing4all
- Type
- Review article
- Journal
- Stem Cell Research and Therapy
- Volume
- 15
- No. of pages
- 17
- ISSN
- 1757-6512
- Publication date
- 23.05.2024
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Medicine (miscellaneous), Molecular Medicine, Biochemistry, Genetics and Molecular Biology (miscellaneous), Cell Biology
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Electronic version(s)
-
https://doi.org/10.1186/s13287-024-03757-6 (Access:
Open)