Development of living cell microarrays using non-contactmicropipette printing

authored by

Rebecca Jonczyk, Suna Timur, Thomas Scheper, Frank Stahl

Abstract

During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue^® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.

Organisation(s)

Institute of Technical Chemistry

External Organisation(s)

Ege University

Type

Article

Journal

Journal of biotechnology

Volume

217

Pages

109-111

No. of pages

3

ISSN

0168-1656

Publication date

19.11.2015

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Bioengineering, Applied Microbiology and Biotechnology

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.1016/j.jbiotec.2015.11.013 (Access: Closed)