Analysis and design of stimulus response curves of E. coli

authored by

Andreas Kremling, Anna Goehler, Knut Jahreis, Markus Nees, Benedikt Auerbach, Wolfgang Schmidt-Heck, Öznur Kökpinar, Robert Geffers, Ursula Rinas, Katja Bettenbrock

Abstract

Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.

Organisation(s)

Institute of Technical Chemistry

External Organisation(s)

Technical University of Munich (TUM)
Osnabrück University
Max Planck Institute for Dynamics of Complex Technical Systems
Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI)
Helmholtz Centre for Infection Research (HZI)

Type

Article

Journal

Metabolites

Volume

2

Pages

844-871

No. of pages

28

Publication date

12.11.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism, Biochemistry, Molecular Biology

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.3390/metabo2040844 (Access: Open)
https://doi.org/10.15488/1554 (Access: Open)