Selective cleavage of the HIV-1 TAR-RNA with a peptide-cyclen conjugate

verfasst von
Katrin Michaelis, Markus Kalesse
Abstract

Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.

Organisationseinheit(en)
Institut für Organische Chemie
Typ
Artikel
Journal
Angewandte Chemie - International Edition
Band
38
Seiten
2243-2245
Anzahl der Seiten
3
ISSN
1433-7851
Publikationsdatum
28.07.1999
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Katalyse, Chemie (insg.)
Ziele für nachhaltige Entwicklung
SDG 3 – Gute Gesundheit und Wohlergehen
Elektronische Version(en)
https://doi.org/10.1002/(SICI)1521-3773(19990802)38:15<2243::AID-ANIE2243>3.0.CO;2-V (Zugang: Geschlossen)