Velvet domain protein VosA represses the zinc cluster transcription factor ScIB regulatory network for Aspergillus nidulans asexual development, oxidative stress response and secondary metabolism

verfasst von: Karl G. Thieme, Jennifer Gerke, Christoph Sasse, Oliver Valerius, Sabine Thieme, Razieh Karimi, Antje K. Heinrich, Florian Finkernagel, Kristina Smith, Helge B. Bode, Michael Freitag, Arthur F. J. Ram, Gerhard H. Braus
Abstract: There is an error in panel D of S8 Fig. Specifically, the upper panel should read ‘SclB-cYFP + nYFP’, not ‘SclA-cYFP + nYFP’. The authors have provided a corrected version here. Supporting information S8 Fig GFP-fusion proteins of SclB are functional and phosphorylated and Bi-FC controls are negative. A) Strains expressing SclB either N- or C-terminally tagged with sGFP in ΔsclB background, ΔsclB and wildtype (WT) were point inoculated on solid MM and grown for 4 days in light. B) SclB-GFP and GFP-SclB fusion proteins expressed under native promoter are visualized in a western hybridization assay employing an α-GFP antibody (GFP) and Ponceau staining as loading control (Pnc). The black arrow indicates bands corresponding to full-length fusion proteins (in silico prediction 87.46 kDa). C) Protein crude extracts of GFP-SclB grown vegetatively were mixed with phosphatase inhibitor cocktail (-/PhoI), with Lambda phosphatase (λ/-), or Lambda phosphatase and phosphatase inhibitor cocktail (λ/PhoI). A control sample was left untreated (-/-). A subsequent western hybridization assay employing α-GFP antibody visualizes protein bands. D) Two strains, either expressing sclB::cyfp and the free second half of the split YFP (nyfp; upper part), or free cyfp and rcoA::nyfp (lower part), under control of a bi-directional nitrate promoter were constructed. Strains were inoculated in liquid MM and analyzed with fluorescence microscopy after 36 h at 30°C. (TIF).
Externe Organisation(en): Georg-August-Universität Göttingen
Typ: Artikel
Journal: PLoS Genetics
Band: 14
ISSN: 1553-7404
Publikationsdatum: 25.07.2018
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Ökologie, Evolution, Verhaltenswissenschaften und Systematik, Molekularbiologie, Genetik, Genetik (klinisch), Krebsforschung
Ziele für nachhaltige Entwicklung: SDG 3 – Gute Gesundheit und Wohlergehen
Elektronische Version(en): https://doi.org/10.1371/journal.pgen.1007511 (Zugang: Offen)
https://doi.org/10.1371/journal.pgen.1007638 (Zugang: Offen)