Detection of feruloyl- and cinnamoyl esterases from basidiomycetes in the presence of interfering laccase

verfasst von

Paul Haase-Aschoff, Diana Linke, Ralf G. Berger

Abstract

Little is known on basidiomycete sources of feruloyl esterases (FAEs), although many wood-rotting representatives of these fungi typically grow on feruloyl-rich substrates. A major reason is that the almost ubiquitous presence of laccases interferes with the detection of FAE activity. Laccases polymerize the liberated ferulic acid (FA). in situ, thus detracting the product of enzymatic hydrolysis from its detection. A rapid HPLC-UV method was developed to detect the loss of FA, but also to quantify the hydrolysis of FA esters. The method allows at the same time to evaluate the substrate specificity of a FAE. Forty one basidiomycetes were tested for their FAE activities, and 25 out of the set were positive. The basidiomycetes hydrolyzing cinnamates with the highest conversion rates were. Auricularia auricula-judae and. Marasmius scorodonius. Moreover, a new FAE inducer, the nonionic detergent Tween 80, was found. This is the first comprehensive study on basidiomycete sources of FAEs.

Organisationseinheit(en)

Institut für Lebensmittelchemie

Typ

Artikel

Journal

Bioresource technology

Band

130

Seiten

231-238

Anzahl der Seiten

8

ISSN

0960-8524

Publikationsdatum

20.12.2012

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Bioengineering, Environmental engineering, Erneuerbare Energien, Nachhaltigkeit und Umwelt, Abfallwirtschaft und -entsorgung

Ziele für nachhaltige Entwicklung

SDG 7 – Erschwingliche und saubere Energie

Elektronische Version(en)

https://doi.org/10.1016/j.biortech.2012.12.039 (Zugang: Unbekannt)