Correlative imaging and quantification of the tumor microenvironment of triple-negative-breast-cancer using lightsheet microscopy, scanning laser optical tomography, and TPEF
- verfasst von
- Hannes Kamin, Lena Nolte, Jochen Maurer, Elmar Stickeler, Dag Heinemann, Alexander Heisterkamp, Sonja Johannsmeier, T. Ripken
- Abstract
The triple-negative breast cancer is an aggressive subtype that has a high rate of relapse and a poor five-year survival rate. The Tumor Microenviroment influences the behaviors such as proliferation, migration and the formation of metastasis. We present the complementary imaging and subsequent analysis of the Tumor Microenviroment using Lightsheet Microscopy, Scanning Laser Optical Tomography and TPEF. The samples were imaged in different size and resolution scales and the three-dimensional information is obtained using the SHG-signal of collagen and fluorescence of labeled key markers. The results presented here may help to create a patient specific model of breast cancer.
- Organisationseinheit(en)
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AG Phytophotonik
Hannoversches Zentrum für Optische Technologien (HOT)
Institut für Quantenoptik
- Externe Organisation(en)
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Laser Zentrum Hannover e.V. (LZH)
Rheinisch-Westfälische Technische Hochschule Aachen (RWTH)
NIFE- Niedersächsisches Zentrum für Biomedizintechnik, Implantatforschung und Entwicklung
- Typ
- Audiovisuelle Veröffentlichung
- Publikationsdatum
- 06.03.2023
- Publikationsstatus
- Veröffentlicht
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
https://doi.org/10.1117/12.2648373 (Zugang:
Geschlossen)
