Macrophage entrapped silica coated superparamagnetic iron oxide particles for controlled drug release in a 3D cancer model
- verfasst von
- Sami Ullah, Katja Seidel, Sibel Türkkan, Dawid Peter Warwas, Tatyana Dubich, Manfred Rohde, Hansjörg Hauser, Peter Behrens, Andreas Kirschning, Mario Köster, Dagmar Wirth
- Abstract
Targeted delivery of drugs is a major challenge in treatment of diverse diseases. Systemically administered drugs demand high doses and are accompanied by poor selectivity and side effects on non-target cells. Here, we introduce a new principle for targeted drug delivery. It is based on macrophages as transporters for nanoparticle-coupled drugs as well as controlled release of drugs by hyperthermia mediated disruption of the cargo cells and simultaneous deliberation of nanoparticle-linked drugs. Hyperthermia is induced by an alternating electromagnetic field (AMF) that induces heat from silica-coated superparamagnetic iron oxide nanoparticles (SPIONs). We show proof-of-principle of controlled release by the simultaneous disruption of the cargo cells and the controlled, AMF induced release of a toxin, which was covalently linked to silica-coated SPIONs via a thermo-sensitive linker. Cells that had not been loaded with SPIONs remain unaffected. Moreover, in a 3D co-culture model we demonstrate specific killing of associated tumour cells when employing a ratio as low as 1:40 (SPION-loaded macrophage: tumour cells). Overall, our results demonstrate that AMF induced drug release from macrophage-entrapped nanoparticles is tightly controlled and may be an attractive novel strategy for targeted drug release.
- Organisationseinheit(en)
-
Institut für Organische Chemie
Institut für Anorganische Chemie
- Externe Organisation(en)
-
Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)
Medizinische Hochschule Hannover (MHH)
- Typ
- Artikel
- Journal
- Journal of controlled release
- Band
- 294
- Seiten
- 327-336
- Anzahl der Seiten
- 10
- ISSN
- 0168-3659
- Publikationsdatum
- 28.01.2019
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Pharmazeutische Wissenschaften
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
http://hdl.handle.net/10033/621629 (Zugang:
Offen)
https://doi.org/10.1016/j.jconrel.2018.12.040 (Zugang: Geschlossen)