Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings
- verfasst von
- B. Rehbock, D. Ganßer, R. G. Berger
- Abstract
2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.
- Organisationseinheit(en)
-
Institut für Lebensmittelchemie
- Typ
- Artikel
- Journal
- Food chemistry
- Band
- 63
- Seiten
- 161-165
- Anzahl der Seiten
- 5
- ISSN
- 0308-8146
- Publikationsdatum
- 30.10.1998
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Analytische Chemie, Lebensmittelwissenschaften
- Ziele für nachhaltige Entwicklung
- SDG 3 – Gute Gesundheit und Wohlergehen
- Elektronische Version(en)
-
https://doi.org/10.1016/S0308-8146(98)00042-9 (Zugang:
Unbekannt)