Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings

verfasst von
B. Rehbock, D. Ganßer, R. G. Berger
Abstract

2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.

Organisationseinheit(en)
Institut für Lebensmittelchemie
Typ
Artikel
Journal
Food chemistry
Band
63
Seiten
161-165
Anzahl der Seiten
5
ISSN
0308-8146
Publikationsdatum
30.10.1998
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Analytische Chemie, Lebensmittelwissenschaften
Ziele für nachhaltige Entwicklung
SDG 3 – Gute Gesundheit und Wohlergehen
Elektronische Version(en)
https://doi.org/10.1016/S0308-8146(98)00042-9 (Zugang: Unbekannt)