Sunlight-Activated Propidium Monoazide Pretreatment for Differentiation of Viable and Dead Bacteria by Quantitative Real-Time Polymerase Chain Reaction

verfasst von

Xing Xie, Siwen Wang, Sunny C. Jiang, Janina Bahnemann, Michael R. Hoffmann

Abstract

Polymerase chain reaction (PCR)-based methods have been developed and increasingly used for rapid and sensitive detection of pathogens in water samples to better protect public health. A propidium monoazide (PMA) pretreatment can help to differentiate between viable and dead cells, but the photoactivation of PMA normally requires the use of an energy-consuming halogen light, which is not suitable for off-the-grid applications. Herein, we investigate sunlight as an alternative light source. Our results suggest that sunlight can successfully activate PMA, and the sunlight-activated PMA pretreatment can effectively reduce the amplification of DNA derived from dead cells in PCR assays. Potentially, a sunlight-activated PMA pretreatment unit can be integrated into a lab-on-a-chip PCR device for off-the-grid microbial detection and quantification.

Organisationseinheit(en)

Institut für Technische Chemie

Externe Organisation(en)

University of California at Irvine
California Institute of Technology (Caltech)

Typ

Artikel

Journal

Environmental Science & Technology Letters

Band

3

Seiten

57-61

Anzahl der Seiten

5

Publikationsdatum

05.01.2016

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Ökologie, Umweltchemie, Gesundheit, Toxikologie und Mutagenese, Umweltverschmutzung, Abfallwirtschaft und -entsorgung, Gewässerkunde und -technologie

Ziele für nachhaltige Entwicklung

SDG 3 – Gute Gesundheit und Wohlergehen

Elektronische Version(en)

https://doi.org/10.1021/acs.estlett.5b00348 (Zugang: Geschlossen)